Bookshelf The Raman Non-destructive AZoM. 1. Advantages and disadvantages of Raman spectroscopy. Non-linear Raman and chiral Raman have powerful but niche uses. CRC Press; 2001. Resonance Raman) which increases the effect. Data were then pre-processed using Savitzky-Golay first or second derivative, SNV, multiplicative scatter correction, and mean center or autoscale. Molecules with functional groups that have strong dipoles display strong peaks in the IR, whereas functional groups that have weak dipoles and readily undergo a change in polarizability display strong peaks in . Thus, Raman spectroscopy has several advantages of mid- or near-IR spectrometers: 2021;217(4):58. These include a Raman microscope coupled to an automated plate reader to support early-stage scale-down conditions [70], an academic study that used in-line viscosity and off-line Raman measurements as input into a Monad model of CHO cell metabolism, [71] and an off-line time-gated Raman/SERS microscopy approach coupled to a custom microwell plate for E. coli fermentations [20]. Bethesda, MD 20894, Web Policies during vibration. J Pharm Innov. Biotechnol Prog. This site needs JavaScript to work properly. Anal Bioanal Chem 414, 969991 (2022). Our 2016 paper described the variety of sampling probe geometries in more detail [18]. Chen MC, Lord RC, Mendelsohn R. Laser-excited Raman spectroscopy of biomolecules. In another approach, the authors generated two off-line pH models from lactate and pCO2 values. 1970;51(2):20313. [78] showed success in Raman-based monitoring of glucose, lactate, glutamate, glutamine, and ammonium during the cultivation of T-cells derived from human donors. J Pharm Biomed Anal. As part of the PAT strategy, Raman spectroscopy offers many benefits and is used successfully in bioprocessing from single-cell analysis to cGMP process control. Berlin: Springer; 2010. For process or in-line sampling applications, fiber optic probes can be inserted directly in the process. The growing role of PAT in bioprocessing is supported by industry, regulatory bodies, and collaborative working groups as noted in an earlier section of this paper. We interviewed Prof. Yuki Ozaki, professor emeritus and a university fellow at Kwansei Gakuin University in Japan, about his work in this field. Can be used with solids, liquids or gases. A reflector at the end of the flow path was used to focus light back into the flow path. However, it should be noted that publications in downstream have been limited until recently. Article J Chem Phys. A recent mini-review by Bell et al. Table 1 gives an overview of bioreactors, Raman probe equipment, and sterilization protocols for the scales often used in upstream bioprocessing. Raman optical activity is used to understand chirality, with applications in biomolecules including mAbs [38, 39]. The second is the establishment of application of NIR spectroscopy to physical chemistry (8). 2023. [cited 2017 Jan 13]. One can meet some of them at the SciX conference. 2020;124(1):5060. Transmitted photons were focused back into the cuvette using a concave mirror on the opposite side of the cuvette from the non-contact optic. Angew Chem Int Ed. Speed is crucial in the analysis because runtimes need to be as. Li M, Ebel B, Chauchard F, Gudon E, Marc A. The existing issues of spectroscopic imaging are the speed of the imaging measurement, the ability to image materials with roughness, and the analysis of depth direction. PAT enables measurement of physical instabilities such as aggregation or chemical alterations including post-translational modifications or cross-linking which can complement stabilization formulation strategies [92]. Chen MC, Lord RC, Mendelsohn R. Laser-excited Raman spectroscopy of biomolecules. When I was a PhD student of Osaka University, I investigated resonance Raman spectroscopy of hemeproteins at the Institute for Protein Research of Osaka University. These studies support the continuation of this research with the eventual goal of automated amino acid monitoring and control. 2004;108(3):82740. Another possible reason is the difficulty in finding an application in which Raman provides a cost-effective benefit over existing measurements such as UVVis and then proving out the technology. One approach, from Santos et al. The new control strategy had minimal effect on VCC, viability, glycosylation, and charge variance. Edsall JT. A Raman microscope can also be integrated with microtiter plates for automated higher throughput analysis of small volume samples (<1mL). The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. Raman spectroscopy has considerable advantages over IR in inorganic systems due to two reasons. Stelling A, Salzer R, Kirsch M, Sobottka SB, Geiger K, Koch E, Schackert G, Steiner G. Anal Bioanal Chem. Li CH, Li T. Application of vibrational spectroscopy to the structural characterization of monoclonal antibody and its aggregate. From your perspective, what are the applications of Raman imaging that highlight its advantages over IR and NIR imaging? Additionally, Raman spec-troscopy is typically able to achieve a better resolution.16 Due to its advantages over current techniques, there is a huge interest in developing Raman spectroscopy and imaging Santos RM, Kessler J-M, Salou P, Menezes JC, Peinado A. called the Principle of mutual exclusion. Can be swamped by fluorescence from some materials. 2020;117(1):14656. Cordero E, Korinth F, Stiebing C, Krafft C, Schie IW, Popp J. Success in upstream monitoring applications and a deeper knowledge of Ramans capabilities support the extension of Raman spectroscopy to downstream bioprocessing. Raman spectroscopy can be combined with optical confocal microscopy to generate a new analytic technique called Raman microscopy. Ozaki has published six textbooks and approximately 1000 papers with an H-index of 72. For infrared spectroscopy solids must be ground into KBr pellets or with nujol to form a mull. Raman spectroscopy has a number of advantages over other analysis techniques. Cross-instrument transferability also occurs when scaling from lab-to-process. Esmonde-White K. Raman spectroscopy as a tool for analytical quality control in a hospital environment [Internet]. Available from: http://www.americanpharmaceuticalreview.com/Featured-Articles/352447-Biopharmaceutical-Process-Model-Evolution-Enabling-Process-Knowledge-Continuum-from-an-Advanced-Process-Control-Perspective/. Raman Spectra of amino acids and related substances III. Collectively, these Raman and UV-resonance Raman studies show the breadth of possible applications for integrating Raman spectroscopy into higher-order structure, formulations, and therapeutic protein composition studies. 2023 MJH Life Sciences and Spectroscopy Online. Bioprocess engineering or bioprocessing describes how the principles of biotechnology get translated into creating useful objects [7]. Over the last decade, Raman spectroscopy has gained more and more interest in research as well as in clinical laboratories. Biotechnol Prog. PAT provides real-time understanding which helps to manage risk throughout a biopharmaceutical products lifecycle. Principle of Raman Spectroscopy 2. Analysis of chemometric models applied to Raman spectroscopy for monitoring key metabolites of cell culture. After analyzing the data, the risk factors were reassessed, and scale and base powder remained the factor with the highest risk priority number (RPN). 2004;22(11):13938. In-line Raman studies described below further establish the use of Raman for measuring major biochemical process parameters such as glucose, lactate, glutamine, and glutamate. Traditional mid-IR spectrophotometers have a spectral range of 4000-400 cm 1.Far-IR spectrophotometers have a spectral range of 500-50 cm 1, but are not as easy to use due to less than optimum optics. The results indicate that the experimental protocol could be used to assess glycation, with possible Raman glycation markers at~1040cm1 and 1121cm1. Product Marketing Manager, from Thermo Fisher Scientific about the new CleanMill Broad Ion Beam System. Inset: Distribution of the literature on FIA-vibrational spectrometry as a function of the detection techniques employed. Bioengineering. Optical photons can interact with molecular vibrations. Time-resolved or SERDS may provide a faster means of reducing fluorescence, and these approaches show promise in reducing fluorescence in Raman spectra of biological materials [19, 20]. [57] reporting on cross-product transferability. For those parameters, the authors hypothesized that the accuracy of the off-line measurement affected the glutamate model and collinearity with other cell-related parameters, and a lack of early-stage data affected the product concentration model. 8600 Rockville Pike Since Raman instruments typically use a laser in the visible/NIR region, flexible silica fiber optic cables can be used to transmit energy, excite the molecule and collect scattered radiation. Biotechnol Bioeng. 2004;16(2):S25-37. Figure permissions: figure reused from Kozma et al. 2019;108(6):194452. Front Psychiatry. Sundberg SA. This page titled 19.9: Advantages and Disadvantages is shared under a CC BY-NC-SA license and was authored, remixed, and/or curated by Dissemination of IT for the Promotion of Materials Science (DoITPoMS). Raman spectra of ten aqueous transfer RNAs and 5S RNA. Ozaki is the winner of the 2020 Charles Mann Award for Applied Raman Spectroscopy to be given at the 2020 SciX conference for his Raman work in biomedical applications. Based on inelastic scattering of a monochromatic excitation source Raman spectra were first compared to off-line pH values across the entire 17-day culture, but they found that full-scale pH was too complex to model. are active in both IR and Raman. Since the 1980s there has been an interest in exploring the use of Raman spectroscopy for biomedical applications requiring in situ measurements where no sample preparation or manipulated pathlength of samples is permitted. Wei H, Mo J, Tao L, Russell RJ, Tymiak AA, Chen G, et al. Raman spectroscopy not only identifies the analyte in a general way and reveals its characteristics, but brings out more hidden data such as such as the crystalline structure, polymorphous state and phases, in addition to inherent strain or stress on the molecular structure, folding of proteins and the presence of hydrogen bonds. Raman data were preprocessed to truncate the spectral region to 5001700cm1, and a 1st or 2nd derivative, a Savitzky-Golay filter and SNV, was applied. In this review, we present currently ongoing research in different fields of medical diagnostics involving linear Raman spectroscopy and imaging. Biochemistry. Kaschif Ahmed S, Guenard R, Romero-Torres S, Antoniou C. Hybrid model identification for monoclonal antibody production bioreactor a digital twin [Internet]. Andr S, Lagresle S, Hannas Z, Calvosa , Duponchel L. Mammalian cell culture monitoring using in situ spectroscopy: is your method really optimised? Vasko PD, Blackwell J, Koenig JL. In this respect, scientific feasibility assessment in the life sciences benefits from a rich literature history since the 1930s. In-line Raman spectra were collected from seven batches and used to calibrate a PLS model, while samples from the lab and pilot scale were used for model validation. Importantly, these studies measured protein conformation under normal and denaturating conditions and used synthetic polypeptides as models of secondary structures such as -helix or -sheet.

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